New

In3
Projects and initiatives

In3

The in3 project aims to drive the synergistic development and utilisation of in vitro and in silico tools for human chemical and nanomaterial safety assessment. The project focused on differentiation of human induced pluripotent stem cells to toxicologically relevant target tissues including; brain, lung, liver and kidney. The tissues, from the same genetic backgrounds, were exposed to common compounds and the data generated and prediction tools generated were used to develop modernised safety assessment approaches combining cheminformatics, mechanistic toxicology and biokinetics into computational models which can account for donor and tissue specific effects.
03:258 days ago
The Beyond Animal Testing Index
Projects and initiatives

The Beyond Animal Testing Index

The Beyond Animal Testing Index (BATI) was designed after the Access to Medicine Index with the aim to be a transparent, objective and independent benchmark that provide public research organisations and their stakeholders insight in what efforts and contributions they make in the transition to animal free innovation and to provide organisations incentive to learn from and inspire each other with regard to the implementation of research practices without the use of animals for the benefit of science.
02:0021 days ago
Transition beyond animal welfare
Expert interviews

Transition beyond animal welfare

This video explains what the programme TPI (Transition Programme for Innovation without the use of animals) is about.
02:3935 days ago
SCREENED
Projects and initiatives

SCREENED

The European collaborative project SCREENED aims to develop three-dimensional (3D) cell-based in vitro tests to better characterize the effects of endocrine disruptors (EDs) on thyroid gland function. This method will overcome the limitations of existing tests, being more sensitive at low doses of exposure to chemicals, and enabling the prediction of their toxicity on human health in a sex-specific manner. The ambition of the SCREENED project is that these new 3D in vitro tests, as well as the increased knowledge about adverse reactions after exposure to EDs, will be used for regulatory purposes, ultimately to improve human health.
02:2135 days ago
Stichting Proefdiervrij: Collaboration is key
Expert interviews
HealthInnovationPolicy

Stichting Proefdiervrij: Collaboration is key

At Stichting Proefdiervrij (the Dutch society for the replacement of animal testing) we believe that collaboration is essential for the development and implementation of animal-free models. In this video we introduce a few of the ways in which we, as an NGO, collaborate with researchers to reach our goal: the complete replacement of all test on animals
02:0755 days ago
Debate about animal testing
Meeting videos
HealthInnovationPolicy

Debate about animal testing

Animal testing contributes to advances in medicine and science in general. But in recent years people have increasingly questioned research using laboratory animals. The European Union and the Dutch government want to be a forerunner in the development and use of innovations that do not involve animal testing, but how do we want to achieve that? What are the challenges and opportunities for biomedical sciences? How do we accelerate the transition towards animal-free innovation? And what does this mean for research into better treatments for animals? In this debate Dutch leaders in the field of animal(-free) testing share their thoughts and opinions.
01:2756 days ago
PrecisionTox
Projects and initiatives

PrecisionTox

In order to better protect human health and the environment from harmful chemicals, the European chemical agency (ECHA) pursues the objective of "zero tolerance" on non-compliance of chemical registration applications. In this video, scientists of the PrecisionTox project - an EU-funded project aiming to accelerate chemical safety assessment with 3Rs compliant models - explain how New Approach Methods (NAMs) provide rich biological data that can help close the data gap to increase acceptance of chemicals dossier while reducing, replacing and refining animal experimentation.
02:092 months ago
New approaches for cancer hazard assessment
Innovation examples

New approaches for cancer hazard assessment

Chemical substances are subjected to assessment of genotoxic and carcinogenic effects before being marketed to protect man and the environment from health risks. For cancer hazard assessment, the long-term rodent carcinogenicity study is the current mainstay for the detection of nongenotoxic carcinogens. However, carcinogenicity studies are shown to have prominent weaknesses and are subject to ethical and scientific debate. A transition toward a mechanism-based weight of evidence approach is considered a requirement to enhance the prediction of carcinogenic potential for chemicals. At RIVM, we are working on this alternative approach for cancer hazard assessment, which makes optimal use of innovative (computational) tools and be less animal demanding. For more information, click on the link in the video or read on here (https://doi.org/10.1080/10408444.2020.1841732) and here (https://doi.org/10.1080/10408444.2018.1458818). Contact the expert (https://nl.linkedin.com/in/mirjamluijten)
03:143 months ago
Zebrafish in toxicity testing
Innovation examples

Zebrafish in toxicity testing

Zebrafish are increasingly recognised as a useful model for toxicity testing of chemical substances. Testing strategies are becoming more based on mechanisms of toxicity structured in adverse outcome pathways describing the chain of events leading to toxicity or disease. Using a battery of dedicated in vitro and in silico assays, insight can be gained in how exposure leads to disease. For certain diseases it is known that toxicity relies on the interaction between different organs and cell types, which requires research on whole organisms in addition to simple in vitro models. The zebrafish is considered a valuable whole organism model in a mechanism-based testing strategy. At RIVM, the zebrafish embryo model is used for testing the effect of chemical substances on several adverse outcomes and diseases. For more information see: https://ehp.niehs.nih.gov/doi/10.1289/EHP9888; https://doi.org/10.3390/ijerph18136717; www.linkedin.com/in/harm-heusinkveld
03:013 months ago
Animal-free computational modelling for prevention of human chemical-induced neural tube defects
Innovation examples

Animal-free computational modelling for prevention of human chemical-induced neural tube defects

Animal-free methods for human chemical safety assessment are promising tools for the reduction of animal testing. However, these methods only measure a small aspect of biology compared to an in vivo test. The reductionist nature of these methods thus limits their individual application in the regulatory arena of chemical risk assessment. Ontologies can be used to describe human biology, and delineate the basis of adverse outcome pathway networks that describe how chemical exposures may lead to adverse health effects. This pathway description can then help to select animal-free in vitro and in silico methods, comprehensively covering the network. The comprehensiveness of this approach, firmly rooted in human biology, is expected to facilitate regulatory acceptance of animal-free methods. As an example, this video zooms in on the development of a computational model for neural tube development, an aspect of human development that is especially vulnerable to chemical disruption. This research is part of the ONTOX project (https://www.ontox-project.eu). For more information on the concept of the Virtual Human, click here (https://doi.org/10.1016/j.cotox.2019.03.009.).
03:054 months ago
Developmental neurotoxicity testing using stem cells
Innovation examples

Developmental neurotoxicity testing using stem cells

Children should grow up in a safe and healthy environment. Disruption of brain development may have enormous impact on future life and might result in disorders such as ADHD or cognitive decline. The effect of compound exposure on the developing brain is largely unknown, since in the current regulatory test procedures in experimental animals effects on the brain are rarely investigated and human relevance of these animal models is under debate. Researchers at RIVM are developing a cell model based on human stem cells that mimics a small part of the developing brain. This method is human-relevant, animal-free, and based on mechanistic knowledge of human biology and physiology of brain development. The model can be an important component in a testing strategy to test the safety of chemicals and pharmaceuticals on the developing brain.
02:275 months ago
Transition Project towards Animal-free Innovations
Projects and initiatives

Transition Project towards Animal-free Innovations

Animal-free innovations are emerging at a fast pace. TPI Chair Daniela Salvatori, and TPI ambassadors Jeffrey Beekman and Elly Hol, explain why animal-free innovations are important and how TPI supports researchers in finding or developing animal-free methods for their research. They call for collaboration.
02:156 months ago